🎉

Thank you for being part of the Doulix journey!

After many amazing years, Doulix will close on November 7, 2025.
We deeply thank all our users for their trust and support.
For future plasmid-related services and information, please visit pSEVA. 💙

Toolbox

From designing an inducible promoter to fabricating an adeno-associated virus for gene knock-down, Doulix offers you a complete set of tools for your synthetic biology application.

SureVector Wizard

Upload your FASTA file and get dsDNA fragments optimized to run your SureVector assembly in-house.

Let's start

In-Fusion Wizard

Upload your FASTA file and get dsDNA fragments optimized to run your InFusion assembly in-house.

Let's start

Gibson Wizard

Upload your FASTA file and get dsDNA fragments optimized to run your Gibson assembly in-house.

Let's start

Cloning Wizard

Upload your sequence file and DOULIX will guide you through gene assembling process.

Let's start

Build

All the tools you need for DNA manufacturing:
linear dsDNA fragments from 0.25 to 2.5 kbp,
cloned fragments from 2.5 to 7.5 kbp, and
multi-gene constructs from 7.5 to 35 kbp

up to 35 kbp

Let's start

Design

All the tools you need for DNA design:
Sequence editor for designing DNA standard parts
Construct Designer to assemble DNA parts into constructs
Start from scratch or choose one of our dedicated tools.

Let's start

BioBrick designer

Are you part of an iGEM team? Create and synthesize your BioBrick with DOULIX and use the registry ID assigned to you by the iGEM committee!

max length < 25 kbp

Let's start

Backbone

Import your FASTA, remove the MCS and create your custom backbone for your routine applications. Use it for all your DOULIX designs.

max length < 15 kbp

Let's start

Bulk upload

Bulk upload your DNA parts (aka biomodules) as multi- .gb or .fasta file.

max length < 25 kbp

Let's start

Page 1 of 3