The power of AI to automate fragments and primers design
Test more than 10.000 fragments in less than 4 minutes
Yes, our AI-powered assembly wizard is included in your free account
You can refine both assembly and PCR parameters to fit your needs
dsDNA fragments comply with the most stringent manufacturing criteria
90% success rate
Tested on a dataset of 482 plasmids, our AI had a 90% success rate
How does it work?
DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard builds and validates the optimal set of fragments to run your assembly in-house.
What is Gibson Assembly?
The SGI's Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct. Gibson Assembly efficiently and precisely fuses linear dsDNA fragments by recognizing 40-60bp overlaps at their ends.
Reference: Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO (2009). "Enzymatic assembly of DNA molecules up to several hundred kilobases". Nature Methods. 6 (5): 3 (see reference).
Disclaimer: GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.