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Gibson Wizard

Upload your sequence and let Doulix's AI compute the fragments

What is Gibson Assembly?
The SGI's Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.
Gibson Assembly efficiently and precisely fuses linear dsDNA fragments by recognizing 40-60bp overlaps at their ends.

How DOULIX's Wizard work?
DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard build and validate fragments set that can be used to assemble your construct.

Gibson Wizard

From design to synthesis in one click!
Unlike any other computer-aided design tool, Doulix allows you to completely focus on designing. With DOULIX's Gibson Wizard now you can harness the power of Gibson assembly with few clicks.
Just upload your complete sequence in a single FASTA format file and DOULIX’s smart algorithm will compute the perfect fragments and primers for you to run your Gibson Assembly reaction in house. Once you've done with the fragments design, ask for a non-binding quote to receive dsDNA fragments starting from only 0.12 euro/bp.

Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO (2009). "Enzymatic assembly of DNA molecules up to several hundred kilobases". Nature Methods. 6 (5): 3 (see reference).

GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.

By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.
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