Upload your sequence and let Doulix's AI compute the fragments
What is Gibson Assembly?The SGI's Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct. Gibson Assembly efficiently and precisely fuses linear dsDNA fragments by recognizing 40-60bp overlaps at their ends.
How DOULIX's Wizard work?DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard build and validate fragments set that can be used to assemble your construct.