uses cookies to ensure you get the best experience on our website. Learn more

SureVector Wizard

The power of AI to automate fragments and primers design
Test more than 10.000 fragments in less than 4 minutes

SureVector Wizard

Key benefits


Yes, our AI-powered assembly wizard is included in your free account

Fully customizable

You can refine both assembly and PCR parameters to fit your needs


dsDNA fragments comply with the most stringent manufacturing criteria

90% success rate

Tested on a dataset of 482 plasmids, our AI had a 90% success rate

How does it work?

DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard builds and validates the optimal set of fragments to run your assembly in-house.
With DOULIX's SureVector Wizard now you can harness the power of SureVector assembly with few clicks. Just upload your complete sequence in a single FASTA format file and DOULIX’s smart algorithm will compute the perfect fragments and primers for you to run your SureVector Assembly reaction in house.

What is SureVector Assembly?

The Agilent's SureVector Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Agilent 's SureVector cloning kit uses a unique enzyme mix that allows a gene-of-interest to be cloned into a fully customizable vector backbone in just 20 minutes. The SureVector single-tube reaction is virtually hands-free, making it the perfect choice for HT-cloning and automation. Just choose which modules to include, combine them in a single tube and transform into XL1-Blue Supercompetent cells.
Once you've done with the fragments design, ask for a non-binding quote to receive dsDNA fragments starting from only 0.12 euro/bp.

Disclaimer: SureVector® is a registered trademark of Agilent Technologies, Inc.

In collaboration with:

SureVector Wizard-logo
By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.
Create your free account