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In-Fusion Wizard

The power of AI to automate fragments and primers design
Test more than 10.000 fragments in less than 4 minutes
In-Fusion Wizard

Key benefits

Free

Yes, our AI-powered assembly wizard is included in your free account

Fully customizable

You can refine both assembly and PCR parameters to fit your needs

Synthesis-ready

dsDNA fragments comply with the most stringent manufacturing criteria

90% success rate

Tested on a dataset of 482 plasmids, our AI had a 90% success rate

How does it work?

DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard builds and validates the optimal set of fragments to run your assembly in-house.

What is In-Fusion Assembly?

The Clonetech's In-Fusion Assembly is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments by recognizing 15-bp overlaps at their ends, without the need for restriction enzyme digestion or compatible restriction sites. The efficiency of In-Fusion cloning procedure is over 95% and just one-pot quick reaction allows you to recover your final construct.

Disclaimer:
IN-FUSIONĀ® is a registered trademark of ClonTech Laboratories, Inc.
By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.
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