Upload your sequence and let Doulix's AI compute the fragments
What is In-Fusion Assembly?The Clonetech's In-Fusion Assembly is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments by recognizing 15-bp overlaps at their ends, without the need for restriction enzyme digestion or compatible restriction sites. The efficiency of In-Fusion cloning procedure is over 95% and just one-pot quick reaction allows you to recover your final construct.
How DOULIX's Wizard work?DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard build and validate fragments set that can be used to assemble your construct.