The power of AI to automate fragments and primers design
Test more than 10.000 fragments in less than 4 minutes
Yes, our AI-powered assembly wizard is included in your free account
You can refine both assembly and PCR parameters to fit your needs
dsDNA fragments comply with the most stringent manufacturing criteria
90% success rate
Tested on a dataset of 482 plasmids, our AI had a 90% success rate
How does it work?
DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard builds and validates the optimal set of fragments to run your assembly in-house.
What is In-Fusion Assembly?
The Clonetech's In-Fusion Assembly is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments by recognizing 15-bp overlaps at their ends, without the need for restriction enzyme digestion or compatible restriction sites. The efficiency of In-Fusion cloning procedure is over 95% and just one-pot quick reaction allows you to recover your final construct.Disclaimer:
IN-FUSION® is a registered trademark of ClonTech Laboratories, Inc.