Just upload your complete sequence in a single FASTA format file and DOULIX will guide you through gene assembling process. You can choose to have your construct delivered as ready-to-use lyophilized sample or you can choose your cloning method of choice and DOULIX’s smart algorithm will compute the perfect fragments for you to run your assembly reaction in house.
DOULIX’s smart algorithm now covers:
- Scarless Overlap Assembly method for designing the optimal fragments to run any overlap-based assembly method, including GIBSON, SUREVECTOR and INFUSION >
- DNAmate Assembly method for designing the optimal fragments for combinatorial assembly based on the DNAmate protocol>
- SureVector Assembly method specifically designed for Agilent’s Surevector cloning and assembly>
- Golden Gate Assembly method designed to use BsaI type IIS restriction endonuclease to create custom overhangs >