Toolbox
From designing an inducible promoter to fabricating an adeno-associated virus for gene knock-down, Doulix offers you a complete set of tools for your synthetic biology application.

AAV Wizard
Design your AAV vector for gene therapy, knock-down studies and cell engineering. Browse our collection of tissue-specific promoters.
max length < 15 kbp
Backbone
Import your FASTA, remove the MCS and create your custom backbone for your routine applications. Use it for all your DOULIX designs.
max length < 15 kbp
BioBrick cloning with type IIS
Are you part of an iGEM team? Create and synthesize your BioBrick with type IIS-compatible vectors
max length < 25 kbp
BioBrick designer
Are you part of an iGEM team? Create and synthesize your BioBrick with DOULIX and use the registry ID assigned to you by the iGEM committee!
max length < 25 kbp
Biomodule design tools
Biomodules are Doulix design building blocks. Biomodules are linear double-stranded DNA parts. As short as a T7 promoter or as long as an entire metabolic pathway, biomodule are your DNA Lego bricks , you can assemble to build your constructs.

Build
All the tools you need for DNA manufacturing:
linear dsDNA fragments from 0.25 to 2.5 kbp,
cloned fragments from 2.5 to 7.5 kbp, and
multi-gene constructs from 7.5 to 35 kbp

Bulk construct composer
Design hundreds of constructs simultaneously using the Doulix advanced composer. Combine premade and new biomodules to quickly design your constructs.

Bulk upload
Bulk upload your DNA parts (aka biomodules) as multi- .gb or .fasta file.
max length < 25 kbp
CIDAR MoClo Level 0
Design your CIDAR MoClo part acceptor to be used for cloning of standardized genetic modules with MoClo cloning protocols
max length < 15 kbp