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BioBrick cloning with type IIS
Are you part of an iGEM team? Create and synthesize your BioBrick with type IIS-compatible vectorsmax length < 25kb
Design a Biomodule
From simple promoters to polycistronic operons, BIOMODULEs are the building blocks of DOULIX. Start designing now!max length < 25kb
Design a Construct
Design your plasmid from scratch by assembling lab-validated BIOMODULEs or designing your own DNA parts.
Upload your sequence file and DOULIX will guide you through gene assembling process.
Design a pSEVA vector
Design and validate your SEVA plasmid using hundreds of compatible parts and then seamlessly move to synthesis with DOULIX's algorithm.max length < 15kb
Upload your FASTA file and get dsDNA fragments optimized to run your SureVector assembly in-house.
Upload your FASTA file and get dsDNA fragments optimized to run your InFusion assembly in-house.
Upload your FASTA file and get dsDNA fragments optimized to run your Gibson assembly in-house.
Import your plasmids, phagemids or any circular dsDNA vector into DOULIX and start working right away.