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Gene cloning

Cloned fragments synthesis

Doulix custom gene cloning provides assembly and expression ready vectors of unparalleled quality, length and complexity. Order 100% sequence perfect clones with ease and we’ll do the rest, from optimisation to preparation.

  • Inserts of 250 bp – 7.5 kbp
  • 80% and < 20% GC content
  • Complexity as standard
  • From €0.12 per bp.

Our quality promise

All Doulix cloned fragments are 100% sequenced verified with Sanger sequencing and produced to our rigorous laboratory procedures according ISO 9001:2008 standards.

  • 100% sanger sequence verified.
  • Troubleshooting as standard.
  • ISO 9001:2008 production standards.
  • Doulix quality promise or your money back.

The extra touch

Industry leading optimisation tools and an expert lab team means we can tackle even the toughest challenges and provide a quality, expression optimised plasmid. Our AI algorithm, trained on thousands of real world experiments computes multiple factors to optimise assembly, and expression.

  • Troubleshooting as standard.
  • Scarless assembly.
  • Expression optimisation.
  • Design and parts support.

Delivery

Standard turnaround time (TAT) on custom cloning vectors starts from 16 working days days. Of course, depending on complexity TAT may vary accordingly. We estimate delivery during checkout and then keep you up to date in real-time. Meaning you’re ready to start research the moment your samples arrive.

  • Avg. turnaround 21 working days (may vary)
  • Real-time project updates and one-to-one support.
  • Lyophilized DNA options in a range of prep sizes (1 ug, 10 ug, 50 ug, 5 mg)
  • E. coli stab agar and glycerol stock delivery options.
  • Add ons available for one stop shopping
  • Customs and shipping handling included.

Accepted inputs: A, C, G, T, W (weak: A, T), S (strong: C, G ), M (amino: A, C), K (keto: G, T), R (purine: A, G ), Y (pyrimidine: C, T), B (not A), D (not C), H (not G), V (not T), N (any basebase)
Blast BP: 0
Sequence and name fields will be overridden
By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.
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