This placeholder is meant to host a transcription terminator, namely a section of nucleic acid sequence that marks the end of a gene or operon in genomic DNA during transcription. This sequence mediates transcriptional termination by providing signals in the newly synthesized mRNA that trigger processes which release the mRNA from the transcriptional complex.
tCYC1 - Standard terminator (9OCV8EJ)
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“Standard terminator”
This Biomodule encodes for a yeast terminator of the MIAMI Project collection. This terminator was characterized in vivo in S.cerevisiae [1] using the protocol described in [2]. Briefly, the terminator was cloned downstream the MIAMI Standard reporter gene cassette [3] and integrated into S.cerevisiae genome by EasyClone marker-free integration as described in [4]. Terminator’s strength was assessed in vivo over 24 hours and results re-scaled using the standard operating procedure described in [5].
MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.
Reference:
[1] S.cerevisiae CEN.PK2-1C
[2] MIAMI's Standard Operating Procedures
[3] MIAMI - Standard reporter gene cassette
[4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link
[5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader