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MIAMI - Standard reporter gene cassette.

This construct encodes for a standard reporter gene cassette used to characterize promoters, terminator and transcription initiation sites in vivo in S.cerevisiae [1] using the protocol described in [2]. Briefly, the parts can be cloned into the appropriate placeholder of the MIAMI – Standard reporter gene cassette [3] and integrated into S.cerevisiae genome by EasyClone marker-free integration as described in [4]. Promoter’s strength was assessed in vivo over 24 hours and results re-scaled using the standard operating procedure described in [5].

MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.

Reference:
[1] S.cerevisiae CEN.PK2-1C
[2] MIAMI's Standard Operating Procedures
[3] MIAMI - Standard reporter gene cassette
[4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link
[5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader

  • Part ID 81JSVKY
  • Parent -
  • Name MIAMI - Standard reporter gene cassette.
  • Version 1
  • Division SYN
  • Template MIAMI - Promoter characterization cassette
  • Collection MIAMI project
  • Part Status Valid
  • Public check_circle_outline
  • Certified check_circle_outline
  • Length 717
  • Author Jensen Lab – DTU
  • Creation Date Tuesday 13 October 2020 10:02
  • Latest Update Monday 02 November 2020 14:17
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Collaborators:



Author:

Jensen Lab – DTU