Plasmid for the expression of a corn aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene.
Corn is a fluorogenic RNA aptamer developed by Song et al. for live imaging of cells (doi.org/10.1038/nchembio.2477). The aptamer provides fluorescence in presence of the fluorophore DFHO (excitation/emission wavelength: 505/545 nm).

  • Part ID CJG2GUY
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_tRNA::corn_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
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  • Length 3772
  • Author Dario Cecchi
  • Creation Date Tuesday 16 February 2021 14:34
  • Latest Update Wednesday 06 April 2022 11:51