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pDLX_6K100_AH_PT7_tRNA::iSpinach_BBaB0015

Plasmid for the expression of an iSpinach aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. iSpinach is an improved version of the fluorogenic RNA-aptamer spinach far less salt-sensitive and with a much higher thermal stability and it was developed by Autor et al. (doi.org/10.1093/nar/gkw083). The aptamer provides fluorescence in presence of the fluorophore DFHBI or derivatives (excitation/emission wavelength: 447/501 nm).

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.
  • Part ID 6I3ZNYD
  • Parent link 46PKNRL - pDLX_6K100_AH_Ptac_BBaB0034_m…
  • Name pDLX_6K100_AH_PT7_tRNA::iSpinach_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Public check_circle_outline
  • Certified highlight_off
  • Length 3813
  • Author Dario Cecchi
  • Creation Date Tuesday 16 February 2021 14:24
  • Latest Update Wednesday 06 April 2022 11:49