Plasmid for the expression of a dBroccoli aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. dBroccoli is a dimeric version of the fluorogenic RNA-aptamer developed by Filonov et al. (doi.org/10.1021/ja508478x). The aptamer provides fluorescence in presence of the fluorophore DFHBI or derivatives (excitation/emission wavelength: 447/501 nm).

  • Part ID 96LKR2X
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_tRNA::dBroccoli_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Editable check_circle
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  • Length 3836
  • Author Dario Cecchi
  • Creation Date Tuesday 23 March 2021 16:47
  • Latest Update Wednesday 06 April 2022 11:55