pBin backbone

pBin backbone derivates from Agrobacterium tumefaciens Ti plasmid and is meant to carry the cargo to be transfected to the plant host. It contains the left border (LB) and right border (RB) short direct repeats required to initiate the transfer. In addition, it contains ORI and selection markers for effective use in multiple hosts.

MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.

[1] S.cerevisiae CEN.PK2-1C
[2] MIAMI's Standard Operating Procedures
[3] MIAMI - Standard reporter gene cassette
[4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link
[5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader

  • Part ID QEOC0VR
  • Name pBin backbone
  • Alternative Name -
  • Version 1
  • Division SYN
  • Parent Sequence -
  • Collection MIAMI project
  • Part Status Valid
  • Public check_circle
  • Certified check_circle
  • Length 9300
  • Source Strain E. coli
  • Suitable for


  • Registry Code -
  • Contains
  • Created by Vincent Courdavault
  • Created at Tuesday 27 August 2019 20:29
  • Last Edit Tuesday 27 August 2019 21:33