Thank you for being part of the Doulix journey!
After many amazing years, Doulix will close on November 7, 2025. We deeply thank all our users for their trust and support. For future plasmid-related services and information, please visit pSEVA. 💙Gibson Assembly® HiFi 1-Step
SKU: SGI-GA1100 · Manufactured by: CODEX DNA, Inc.
Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix performs three different enzymatic activities in a one-pot reaction:
- single-stranded 3´ overhangs (overlap region) creation
- polymerase fill-in of gaps
- DNA ligation
CODEX DNA’s Gibson Assembly® HiFi 1-Step master mix is the original Gibson Assembly® cloning method, which has been optimized for the scarless joining of up to 5 inserts into a plasmid vector, resulting in DNA constructs with reliably low error rates. This single-tube, isothermal reaction is compatible with fragments ranging from 500 bp to 32 kb and can be completed in 60 minutes or less. Unlike other approaches, CODEX DNA’s Gibson Assembly® process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This proof-reading process results in higher numbers of error-free clones ready for multiple downstream applications.
With over 4,000 citations in the scientific literature [Gibson et al. 2009. Nature Methods], CODEX DNA’s Gibson Assembly® is the method of choice for your DNA assembly.
For less concentrated DNA samples, you may be interested in:
Both the original 2X master mix and 4X high concentration (HC) master mix can assemble multiple DNA fragments with greater than 90% cloning efficiency.