Gibson Assembly® HiFi 1-Step

SKU: SGI-GA1100 · Manufactured by: CODEX DNA, Inc.

Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix performs three different enzymatic activities in a one-pot reaction:

  • single-stranded 3´ overhangs (overlap region) creation
  • polymerase fill-in of gaps
  • DNA ligation
The end result is a double-stranded fully sealed DNA molecule ready for most downstream applications

CODEX DNA’s Gibson Assembly® HiFi 1-Step master mix is the original Gibson Assembly® cloning method, which has been optimized for the scarless joining of up to 5 inserts into a plasmid vector, resulting in DNA constructs with reliably low error rates. This single-tube, isothermal reaction is compatible with fragments ranging from 500 bp to 32 kb and can be completed in 60 minutes or less. Unlike other approaches, CODEX DNA’s Gibson Assembly® process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This proof-reading process results in higher numbers of error-free clones ready for multiple downstream applications.


With over 4,000 citations in the scientific literature [Gibson et al. 2009. Nature Methods], CODEX DNA’s Gibson Assembly® is the method of choice for your DNA assembly.

For less concentrated DNA samples, you may be interested in:

Both the original 2X master mix and 4X high concentration (HC) master mix can assemble multiple DNA fragments with greater than 90% cloning efficiency.