Gibson Assembly® HiFi 1-Step
Manufactured by: SGI-DNA, Inc.
Cat. no: SGI-GA1100
Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix performs three different enzymatic activities in a one-pot reaction:
- single-stranded 3´ overhangs (overlap region) creation
- polymerase fill-in of gaps
- DNA ligation
SGI-DNA’s Gibson Assembly® HiFi 1-Step master mix is the original Gibson Assembly® cloning method, which has been optimized for the scarless joining of up to 5 inserts into a plasmid vector, resulting in DNA constructs with reliably low error rates. This single-tube, isothermal reaction is compatible with fragments ranging from 500 bp to 32 kb and can be completed in 60 minutes or less. Unlike other approaches, SGI-DNA’s Gibson Assembly® process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This proof-reading process results in higher numbers of error-free clones ready for multiple downstream applications.
With over 4,000 citations in the scientific literature [Gibson et al. 2009. Nature Methods], SGI-DNA’s Gibson Assembly® is the method of choice for your DNA assembly.
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