This slot is meant to contain IIS type enzymes for your Golden Gate based cloning method
BsaI with terminal A (QDRCYD6)
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Current choice for
“Upstream IIS enzyme”
BsaI is a type IIS restriction enzymes that recognizes asymmetric DNA sequences and cleave outside of their recognition sequence. Type IIS are used in many cloning protocols, including DNAmate, Golden Gate and MoClo Assembly
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