This slot is intended for C-term tags, such as affinity tags, solubilization tags or reporter genes. This slot is flanked by a BamHI restriction site (GGATCC) at the 5' that encodes for Gly-Ser to allow in-frame fusion protein with the upstream slot (your custom gene).
ECK120010818 terminator (MNKCZEY)
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Current choice for
“C-terminus domain”
Natural transcription terminator for E. coli described having medium terminator strength (doi.org/10.1038/nmeth.2515)
The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.