This placeholder is meant to host a promoter, namely a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA. Promoters contain specific DNA sequences such as response elements that provide a secure initial binding site for RNA polymerase and for proteins called transcription factors that recruit RNA polymerase.

pET-15b T7 promoter + LacO + 5'UTR (QR5CKNO)