The biomodule carries all the elements to drive RNA transcription under the control of naringenin in E. coli. An operator for the TetR-family factor TtgR was evolved to allow the induction of gene expression by naringenin (doi.org/10.1093/nar/gkz772). The coding sequence of the TtgR repressor is driven by a constitutive promoter directed in the opposite direction of the naringenin-inducible promoter Pttg. TtgR gene transcription is interrupted by a transcription terminator and TtgR coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by naringenin.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.