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The biomodule carries all the elements to drive RNA transcription under the control of naringenin in E. coli. An operator for the TetR-family factor TtgR was evolved to allow the induction of gene expression by naringenin ( The coding sequence of the TtgR repressor is driven by a constitutive promoter directed in the opposite direction of the naringenin-inducible promoter Pttg. TtgR gene transcription is interrupted by a transcription terminator and TtgR coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by naringenin.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060.
  • Part ID M3GCO72
  • Name DLX_TtgR-Pttg_v3.0
  • Alternative Name -
  • Version -
  • Division -
  • Parent Sequence -
  • Collection ACDC project
  • Part Status Valid
  • Public check_circle_outline
  • Certified highlight_off
  • Length 986
  • Source Strain -
  • Suitable for
    • E. coli
  • Registry Code -
  • Contains
  • Created by Dario Cecchi
  • Created at Monday 29 June 2020 16:53
  • Last Edit Wednesday 09 December 2020 12:02