Promoter
Ptac (M3GCP5V)
No changes for "Ptac (M3GCP5V)"
AfilI
AflII (239CNE)
No changes for "AflII (239CNE)"
5' untranslated region
RBS BBa_B0034 with 3 nt spacer (QY9CRLN)
No changes for "RBS BBa_B0034 with 3 nt spacer (QY9CRLN)"
NdeI
NdeI (0KNCNP)
No changes for "NdeI (0KNCNP)"
Kpn2I
Kpn2I (24XC78)
No changes for "Kpn2I (24XC78)"
Your Custom gene
N-ter leucine zipper (9DO3447)
No changes for "N-ter leucine zipper (9DO3447)"
BamHI
BamHI (0O5CZ5)
No changes for "BamHI (0O5CZ5)"
C-terminus domain
GS linker (6 aa) (0FB41SG)
No changes for "GS linker (6 aa) (0FB41SG)"
KpnI
KpnI (61MC15)
No changes for "KpnI (61MC15)"
3' untranslated region
CspGFP + 2STOP (2HVJY60)
No changes for "CspGFP + 2STOP (2HVJY60)"
EcoRI
EcoRI (0DXC97)
No changes for "EcoRI (0DXC97)"
Terminator
Multiple STOP codons + terminator BBa_B0015 (6VDC5JO)
No changes for "Multiple STOP codons + terminator BBa_B0015 (6VDC5JO)"
XhoI
XhoI (07YCOD)
No changes for "XhoI (07YCOD)"
Vector Backbone
pDLX_3C100 (M7JCK7K)
No changes for "pDLX_3C100 (M7JCK7K)"
XbaI
XbaI (69EC3Q)
No changes for "XbaI (69EC3Q)"
Just now