pDLX_6K100_AH_PT7_tRNA::Broccoli_BBaB0015

Plasmid for the expression of a Broccoli aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. Broccoli is a fluorogenic RNA-aptamer developed by Filonov et al. (doi.org/10.1021/ja508478x). The aptamer provides fluorescence in presence of the fluorophore DFHBI or derivatives (excitation/emission wavelength: 447/501 nm).