Plasmid for the expression of a baby Spinach aptamer under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. bSpinach is a minimal version of the fluorogenic RNA-aptamer spinach developed by Soni et al. (doi.org/10.1039/C9OB01414D). The aptamer provides fluorescence in presence of the fluorophore DFHBI or derivatives (excitation/emission wavelength: 447/501 nm).

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.
  • Part ID 50IP030
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_bSpinach_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Editable check_circle
  • Public check_circle
  • Certified highlight_off
  • Length 3722
  • Author Dario Cecchi
  • Creation Date Tuesday 16 February 2021 14:39
  • Latest Update Wednesday 06 April 2022 11:51