pDLX_6K100_AH_PT7_tRNA::oBroccoli_BBaB0015

Plasmid for the expression of a orange Broccoli aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. oBroccoli is a fluorogenic RNA aptamer developed by Song et al. for live imaging of cells (doi.org/10.1038/nchembio.2477). The aptamer provides fluorescence in presence of the fluorophore DFHO (excitation/emission wavelength: 505/545 nm).

  • Part ID 3HF87DZ
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_tRNA::oBroccoli_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Editable check_circle
  • Public check_circle
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  • Length 3805
  • Author Dario Cecchi
  • Creation Date Tuesday 23 March 2021 16:47
  • Latest Update Wednesday 06 April 2022 11:54