CIDAR DVA backbone

DVA (also known as DVL0) backbone derived from DVA and harbors 2 BsaI restriction sites at the edges that are instrumental for Goldengate assembly. DVA is meant for Level 0 CIDAR constructs carrying individual Biomodule. DVA derives from pSB1A2. It is a high copy number plasmid carrying ampicillin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). All Biomodules of the CIDAR collection were designed by the Densmore Lab at the Boston University and are provided as they are and solely for research purpose. [Iverson et al., ACS Synthetic Biology, 2015]