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CIDAR DVK backbone

DVK (also known as DVL1) backbone is meant for Level 1 CIDAR constructs carrying multiple biomodules assembled using GoldenGate assembly method. For this reason, DVK does not carry any BsaI or BsmBI restriction site that are usually employed in type-IIS assembly methods. It is a high copy number plasmid carrying kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). All sequences of the CIDAR collection were designed by the Densmore Lab at the Boston University and are provided as they are and solely for research purpose. [Iverson et al., ACS Synthetic Biology, 2015]

  • Part ID MGECWK0
  • Name CIDAR DVK backbone
  • Alternative Name -
  • Version 1
  • Division SYN
  • Parent Sequence -
  • Collection CIDAR MoClo
  • Part Status Valid
  • Public check_circle_outline
  • Certified check_circle_outline
  • Length 2227
  • Source Organism Other/Unknown/Synthetic
  • Suitable for -
  • Registry Code -
  • Contains
  • Created by CIDAR
  • Created at July 24, 2019, 11:19 a.m.
  • Last Edit July 24, 2019, 12:20 p.m.