Gibson Wizard

The power of AI to automate fragments and primers design

Test more than 10.000 fragments in less than 4 minutes

Key benefits

Free

Yes, our AI-powered assembly wizard is included in your free account

Fully customizable

You can refine both assembly and PCR parameters to fit your needs

Synthesis-ready

dsDNA fragments comply with the most stringent manufacturing criteria

90% success rate

Tested on a dataset of 482 plasmids, our AI had a 90% success rate

How does it work?

DOULIX's AI-powered assembly wizard scans your sequence to find suitable overlaps with the proper length, GC content, and Tm. Then, thousands of different linear dsDNA fragments are computed between any two overlaps. Each fragment is designed to avoid secondary structure, homopolymeric stretches and other features that may impair its synthesis. Finally, DOULIX's assembly wizard builds and validates the optimal set of fragments to run your assembly in-house.

What is Gibson Assembly?

The CODEX DNA's Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.
Gibson Assembly efficiently and precisely fuses linear dsDNA fragments by recognizing 40-60bp overlaps at their ends.

Reference:
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO (2009). "Enzymatic assembly of DNA molecules up to several hundred kilobases". Nature Methods. 6 (5): 3 (see reference).

Disclaimer:
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.

In collaboration with:

Upload valid FASTA or Genbank

Or select one of our sample sequence

Construct name*

Description

Labels

Biosafety level*

Level 1

Level 2

By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.

I declare this DNA sequence is compliant with applicable biosecurity provisions as defined by US Select Agents and Toxins List and/or European Union Council Regulation 428/2009.*

Create your free account

First name*

Last name*

Email*

Password*

Your password can’t be too similar to your other personal information.
Your password must contain at least 8 characters.

Password (again)*

I agree with terms and conditions*

I agree with biohazard policy*