The biomodule carries all the elements to drive RNA transcription under the control of T7 RNA polymerase and Isopropyl β-D-1-thiogalactopyranoside (IPTG) in E. coli. The coding sequence of the LacI repressor is driven by the constitutive promoter PlacI directed in the opposite direction of the IPTG-inducible promoter composed of T7 promoter and lacO operator. LacI gene transcription is interrupted by a transcription terminator and LacI coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by T7 RNA polymerase and IPTG. The in vivo expression of the downstream gene can only occur in strains carrying T7 RNA polymerase gene, such as E. coli BL21(DE3).

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.