Upstream IIS enzyme

BsaI with terminal A (QDRCYD6)

No changes for "BsaI with terminal A (QDRCYD6)"

5' hinge

CIDAR MoClo C hinge (M1LCG1M)

No changes for "CIDAR MoClo C hinge (M1LCG1M)"

GOI

lon protease (699CEL6)

No changes for "lon protease (699CEL6)"

3' hinge

CIDAR MoClo D hinge (6XDCLOQ)

No changes for "CIDAR MoClo D hinge (6XDCLOQ)"

Downstream IIS enzyme

BsaI with terminal T (QR5CG8M)

No changes for "BsaI with terminal T (QR5CG8M)"

Backbone

pSEVA181_backbone (MLDCKR6)

No changes for "pSEVA181_backbone (MLDCKR6)"