Promoter
Ptac (M3GCP5V)
No changes for "Ptac (M3GCP5V)"
AFilI
AflII (239CNE)
No changes for "AflII (239CNE)"
5' untranslated region
RBS BBa_B0034 with 3 nt spacer (QY9CRLN)
No changes for "RBS BBa_B0034 with 3 nt spacer (QY9CRLN)"
NdeI
NdeI (0KNCNP)
No changes for "NdeI (0KNCNP)"
N-terminus domain
mTagBFP2_PSO (3YQXY8H)
No changes for "mTagBFP2_PSO (3YQXY8H)"
Kpn2I
Kpn2I (24XC78)
No changes for "Kpn2I (24XC78)"
Your Custom gene
His tag + STOP (239CYE)
No changes for "His tag + STOP (239CYE)"
BamHI
BamHI (0O5CZ5)
No changes for "BamHI (0O5CZ5)"
C-terminus domain
ECK120015440 terminator (Q87C211)
No changes for "ECK120015440 terminator (Q87C211)"
KpnI
KpnI (61MC15)
No changes for "KpnI (61MC15)"
3' untranslated region
P3-32 terminator (M1LCL71)
No changes for "P3-32 terminator (M1LCL71)"
EcoRI
EcoRI (0DXC97)
No changes for "EcoRI (0DXC97)"
Terminator
Random sequence 2 (QDRCDKD)
No changes for "Random sequence 2 (QDRCDKD)"
XhoI
XhoI (07YCOD)
No changes for "XhoI (07YCOD)"
Vector Backbone
pDLX_6K100 (M3GC0NO)
No changes for "pDLX_6K100 (M3GC0NO)"
XbaI
XbaI (69EC3Q)
No changes for "XbaI (69EC3Q)"
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