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T7 promoter K1F

The T7 promoter K1F is an engineered promoter that is specifically bound by the engineered T7 (K1F) RNA polymerase. The DNA sequence to which T7 RNAP binds is determined by a beta-hairpin, known as the specificity loop, which contacts the -12 to -7 bp region of the promoter (Cheetham et al. 1999). Changes to the specificity loop confer the ability to recognize different promoter sequences (Chelliserrykattil et al. 2001, Esvelt et al. 2011).The binding region in the T7 promoter K1F was mutated from CGACTC to ACTATC.

  • Part ID ZVMYVE
  • Name T7 promoter K1F
  • Alternative Name -
  • Version -
  • Division -
  • Parent Sequence -
  • Collection -
  • Part Status Valid
  • Public check_circle_outline
  • Certified highlight_off
  • Length 23
  • Source Organism Other/Unknown/Synthetic
  • Suitable for
    • E. coli
  • Registry Code -
  • Contains
  • Created by Doulix Adm
  • Created at Thursday 05 January 2017 16:42
  • Last Edit Thursday 05 January 2017 16:42
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Doulix Adm