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DLX_LuxR-Plux_v3.0

The biomodule carries all the elements to drive RNA transcription under the control of the quorum sensing molecule N-(3-Oxohexanoyl)-L-homoserine lactone (3OC6) in E. coli. The coding sequence of the LuxR transcription activator is driven by a constitutive promoter directed in the opposite direction of the 3OC6-inducible promoter PLux. LuxR gene transcription is interrupted by a transcription terminator and PLux contains a mutation in -10 sequence (converted from TATAGT to TACTTT) described to enhance gene expression (doi.org/10.1021/acssynbio.6b00367). The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by 3OC6.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.
  • Part ID M7JC1N1
  • Name DLX_LuxR-Plux_v3.0
  • Alternative Name -
  • Version -
  • Division -
  • Parent Sequence -
  • Collection ACDC project
  • Part Status Valid
  • Public check_circle_outline
  • Certified check_circle_outline
  • Length 1125
  • Source Organism -
  • Suitable for
    • E. coli
  • Registry Code -
  • Contains
    • Gene
  • Created by Dario Cecchi
  • Created at Monday 29 June 2020 16:55
  • Last Edit Wednesday 09 December 2020 20:41
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Author:

Dario Cecchi