pDLX_1K100

Backbone of a high copy number plasmid compatible with DNAmate assembly, containing a kanamycin resistance gene and the origin of replication from pUC plasmids. The extremities of the biomodule contain IIs restriction sites for the excision of the insert biomodule to be used in cloning techniques based on Golden Gate, such as DNAmate and MoClo. The backbone was also optimized for cloning using the Gibson assembly protocol.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.