LOCUS pseva264 3761 bp DNA circular UNK 11-SEP-2019 DEFINITION . ACCESSION Q45FX9L VERSION Q45FX9L.1 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers source 1..3761 CDS 89..1171 /standard_name="LacIq" /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ*" /product="LacIq" CDS 89..91 /standard_name="alternative Start codon" /product="alternative Start codon" regulatory 1403..1432 /standard_name="Ptrc" /regulatory_class="promoter" regulatory 1438..1458 /standard_name="lacO" /regulatory_class="promoter" primer_bind complement(1548..1571) /standard_name="F24 primer bind" regulatory 1578..1680 /standard_name="T0" /note="rho-independent transcriptional terminator of phage lambda" /regulatory_class="terminator" CDS 1808..2623 /standard_name="neo" /note="neomycin phosphotransferase" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" /product="neo" rep_origin 2655..2900 /standard_name="OriT" rep_origin 2909..3641 /standard_name="p15A" regulatory 3656..3760 /standard_name="T1 terminator" /regulatory_class="terminator" ORIGIN 1 ttaattaatt gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc 61 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga 121 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc 181 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg 241 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct 301 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg 361 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt 421 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca 481 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc 541 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt 601 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc 661 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca 721 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca 781 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct 841 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt 901 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca 961 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca 1021 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc 1081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 1141 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagcgcg 1201 aattgatctg gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca 1261 ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt 1321 cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg 1381 gcaaatattc tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat 1441 tgtgagcgga taacaatttc acaccctagg ccgcggccgc gcgaattcga gctcggtacc 1501 cggggatcct ctagagtcga cctgcaggca tgcaagcttg cggccgcgtc gtgactggga 1561 aaaccctggc gactagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc 1621 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag 1681 gggtccccaa taattacgat ttaaatttgt gtctcaaaat ctctgatgtt acattgcaca 1741 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 1801 gggtgttatg agccatattc agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa 1861 catggatgcg gatctgtatg gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc 1921 gaccatttat cgtctgtatg gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa 1981 aggcagcgtg gcgaacgatg tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt 2041 tatgccgctg ccgaccatta aacattttat tcgcaccccg gatgatgcgt ggctgctgac 2101 caccgcgatt ccgggcaaaa ccgcgtttca ggtgctggaa gaatatccgg atagcggcga 2161 aaacattgtg gatgcgctgg ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa 2221 ctgcccgttt aacagcgatc gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa 2281 cggcctggtg gatgcgagcg attttgatga tgaacgtaac ggctggccgg tggaacaggt 2341 gtggaaagaa atgcataaac tgctgccgtt tagcccggat agcgtggtga cccacggcga 2401 ttttagcctg gataacctga ttttcgatga aggcaaactg attggctgca ttgatgtggg 2461 ccgtgtgggc attgcggatc gttatcagga tctggccatt ctgtggaact gcctgggcga 2521 atttagcccg agcctgcaaa aacgtctgtt tcagaaatat ggcattgata atccggatat 2581 gaacaaactg caatttcatc tgatgctgga tgaatttttc taataattaa ttggacnnnn 2641 gtccgcgcgt tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt 2701 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac 2761 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg 2821 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct 2881 gctctgcgag gctggccgta ggccggccct agaaatattt tatctgatta ataagatgat 2941 cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct 3001 tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc 3061 ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact 3121 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt 3181 ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg 3241 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg 3301 cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg 3361 aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt 3421 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag 3481 cctatggaaa aacggctttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc 3541 atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac 3601 cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc cggcgcgccc agctgtctag 3661 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag 3721 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t //