MIAMI - Standard template for Terminator testing

This construct is the standard template to test Terminator's strength in vivo in S.cerevisiae [1] developed by the MIAMI consortium using the protocol described in [2]. Briefly, the parts can be cloned into the appropriate placeholder of the MIAMI – Standard reporter gene cassette [3] and integrated into S.cerevisiae genome by EasyClone marker-free integration as described in [4]. Terminator's strength is then assessed in vivo over 24 hours and results are re-scaled using the standard operating procedure described in [5].

MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.

[1] S.cerevisiae CEN.PK2-1C
[2] MIAMI's Standard Operating Procedures
[3] MIAMI - Standard reporter gene cassette
[4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link
[5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader

  • Part ID DXFHLM6
  • Parent -
  • Name MIAMI - Standard template for Terminator testing
  • Version -
  • Division -
  • Template MIAMI - Expression cassette
  • Collection MIAMI project
  • Part Status
  • Editable check_circle
  • Public check_circle
  • Certified check_circle
  • Length 1703
  • Author Jensen Lab – DTU
  • Creation Date Monday 19 July 2021 12:24
  • Latest Update Monday 19 July 2021 12:25