Plasmid for the expression of a mango aptamer (III) flanked by a F30 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. MangoIII is a fluorogenic RNA aptamer developed by Autor et al. for live imaging of cells (doi.org/10.1038/s41467-018-02993-8). The aptamer provides fluorescence in presence of the fluorophore thiazole orange (excitation/emission wavelength: 492/516 nm).

  • Part ID BLMF1CL
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_F30::mangoIII_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Editable check_circle
  • Public check_circle
  • Certified highlight_off
  • Length 3769
  • Author Dario Cecchi
  • Creation Date Tuesday 16 February 2021 14:39
  • Latest Update Wednesday 06 April 2022 11:56