Plasmid for the expression of a red Broccoli (clone 14) aptamer flanked by a tRNALys3 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. rBroccoli14 is a fluorogenic RNA aptamer developed by Filonov et al. (doi.org/10.1038/nchembio.2477). The aptamer provides fluorescence in presence of the fluorophore DFHO (excitation/emission wavelength: 505/545 nm).

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.
  • Part ID 8AZ8AJ5
  • Parent link 6I3ZNYD - pDLX_6K100_AH_PT7_tRNA::iSpin…
  • Name pDLX_6K100_AH_PT7_tRNA::rBroccoli14_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
  • Editable check_circle
  • Public check_circle
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  • Length 3805
  • Author Dario Cecchi
  • Creation Date Tuesday 06 April 2021 16:50
  • Latest Update Wednesday 06 April 2022 11:52