Promoter
Ptac (M3GCP5V)
No changes for "Ptac (M3GCP5V)"
AfilI
AflII (239CNE)
No changes for "AflII (239CNE)"
5' untranslated region
RBS BBa_B0034 with 3 nt spacer (QY9CRLN)
No changes for "RBS BBa_B0034 with 3 nt spacer (QY9CRLN)"
NdeI
NdeI (0KNCNP)
No changes for "NdeI (0KNCNP)"
N-terminus domain
6x His tag (GNOKX1)
No changes for "6x His tag (GNOKX1)"
Kpn2I
Kpn2I (24XC78)
No changes for "Kpn2I (24XC78)"
Your Custom gene
NspGFP (AUBVI1P)
No changes for "NspGFP (AUBVI1P)"
BamHI
BamHI (0O5CZ5)
No changes for "BamHI (0O5CZ5)"
C-terminus domain
GS linker (6 aa) (0FB41SG)
No changes for "GS linker (6 aa) (0FB41SG)"
KpnI
KpnI (61MC15)
No changes for "KpnI (61MC15)"
3' untranslated region
C-ter leucine zipper + 2STOP (7THN4GF)
No changes for "C-ter leucine zipper + 2STOP (7THN4GF)"
EcoRI
EcoRI (0DXC97)
No changes for "EcoRI (0DXC97)"
Terminator
Multiple STOP codons + terminator BBa_B0015 (6VDC5JO)
No changes for "Multiple STOP codons + terminator BBa_B0015 (6VDC5JO)"
XhoI
XhoI (07YCOD)
No changes for "XhoI (07YCOD)"
Vector Backbone
pDLX_6K100 (M3GC0NO)
No changes for "pDLX_6K100 (M3GC0NO)"
XbaI
XbaI (69EC3Q)
No changes for "XbaI (69EC3Q)"
Just now