Plasmid for the expression of a mango aptamer (IV) flanked by a F30 scaffold under the control of T7 RNA polymerase. The plasmid is suitable for transcription both in vitro and in vivo in E. coli strains carrying the T7 RNA polymerase gene. MangoIV is a fluorogenic RNA aptamer developed by Autor et al. for live imaging of cells (doi.org/10.1038/s41467-018-02993-8). The aptamer provides fluorescence in presence of the fluorophore thiazole orange (excitation/emission wavelength: 492/516 nm).

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.
  • Part ID 2NFAAP8
  • Parent link BLMF1CL - pDLX_6K100_AH_PT7_F30::mangoI…
  • Name pDLX_6K100_AH_PT7_F30::mangoIV_BBaB0015
  • Version -
  • Division -
  • Template -
  • Collection ACDC project
  • Part Status
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  • Length 3766
  • Author Dario Cecchi
  • Creation Date Tuesday 23 March 2021 16:54
  • Latest Update Wednesday 06 April 2022 11:56