We use cookies to ensure you get the best experience on our website. Learn more. Learn more

T7 promoter 2

T7 promoter 2 is a variant of WT T7 promoter with reduced promoter strength. The T7 promoter has been shown to be modular, consisting of a 6 bp RNAP-binding region and a 5 bp strength determining region (Cheetham et al. 1999,Bandwar et al. 2002, Rong et al. 1998). Mutations to the strength-determining region alter promoter strength without affecting RNAP specificity (Davidson et al. 2010, Ikeda et al. 1992). Mutation of the strength-determining region from TAGGG to GAGGA results in a 13 fold reduced promoter strength compared to the wild type.

  • Part ID ZN7E1W
  • Name T7 promoter 2
  • Alternative Name -
  • Version -
  • Division -
  • Parent Sequence -
  • Collection -
  • Part Status Valid
  • Public check_circle_outline
  • Certified highlight_off
  • Length 23
  • Source Organism Other/Unknown/Synthetic
  • Suitable for
    • E. coli
  • Registry Code -
  • Contains
  • Created by Doulix Adm
  • Created at Thursday 05 January 2017 16:42
  • Last Edit Thursday 05 January 2017 16:42
Biomodule Seq. arrow_forward

Collaborators:



Author:

Doulix Adm