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U6 promoter

The endogenous U6 promoter normally controls expression of the U6 RNA, a small nuclear RNA (snRNA) involved in splicing, and has been well-characterized (Kunkel et al., 1986; Kunkel and Pederson, 1988; Paule and White, 2000). This particular promoter was chosen to control vector-based expression of shRNA molecules in Mammalian cells (Paddison et al., 2002; Paul et al., 2002) for the following reasons: The promoter is recognized by RNA Polymerase III and controls high-level, constitutive expression of shRNA. The promoter is active in most mammalian cell types. The promoter is a type III Pol III promoter in that all elements required to control expression of the shRNA are located upstream of the transcription start site (Paule and White, 2000).

  • Part ID R1LP2N
  • Name U6 promoter
  • Alternative Name -
  • Version -
  • Division -
  • Parent Sequence -
  • Collection -
  • Part Status Valid
  • Public check_circle_outline
  • Certified highlight_off
  • Length 264
  • Source Strain Mammalian cell
  • Suitable for
    • Mammalian cell
  • Registry Code -
  • Contains
  • Created by Doulix Adm
  • Created at Thursday 05 January 2017 16:42
  • Last Edit Thursday 05 January 2017 16:42