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pEAQ_backbone

pEAQ_backbone derivates from Agrobacterium tumefaciens Ti plasmid and is meant to carry the cargo to be transfected to the plant host. It contains the left border (LB) and right border (RB) short direct repeats required to initiate the transfer. In addition, it contains ORI and selection markers for effective use in multiple hosts.

MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.

Reference:
[1] S.cerevisiae CEN.PK2-1C
[2] MIAMI's Standard Operating Procedures
[3] MIAMI - Standard reporter gene cassette
[4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link
[5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader

  • Part ID Q4NCZWG
  • Name pEAQ_backbone
  • Alternative Name -
  • Version 1
  • Division SYN
  • Parent Sequence -
  • Collection MIAMI project
  • Part Status Valid
  • Public check_circle_outline
  • Certified check_circle_outline
  • Length 5114
  • Source Organism E. coli
  • Suitable for
    • E. coli
    • Other/Unknown/Synthetic
  • Registry Code -
  • Contains
    • Selection Marker / Antibiotic Resistance
    • Transcription regulatory region
    • Gene
  • Created by Vincent Courdavault
  • Created at Thursday 11 July 2019 08:15
  • Last Edit Tuesday 27 August 2019 21:33
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Collaborators:



Author:

Vincent Courdavault