EF-1alpha promoter

F-1? (Elongation Factor-1?) promoter is a strong, constitutive non-viral promoter. The EF-1? gene encodes EF-1? enzyme that catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes. EF-1 ? is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells. The EF-1 ? promoter exhibits a strong activity, higher than that of viral promoters such as SV40 and RSV and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo.